Biofabrication of perfusable liver constructs

Engineering a perfusable 3D human liver platform from iPS cells.

In vitro models of human tissue are crucial to our ability to study human disease as well as develop safe and effective drug therapies. Models of single organs in static and microfluidic culture have been established and shown utility for modeling some aspects of health and disease; however, these systems lack multi-organ interactions that are critical to some aspects of drug metabolism and tox...

Biofabrication of Tissue Scaffolds

Biofabrication Under Fluorocarbon: A Novel Freeform Fabrication Technique to Generate High Aspect Ratio Tissue-Engineered Constructs

Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according to a computer-generated three-dimensional model. The lack of mechanical stability of printed hydrogels hinders the fabrication of high aspect ratio constructs. Here we present submerg...

Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy

A hybrid 3D bioprinting approach using porous microscaffolds and extrusion-based printing method is presented. Bioink constitutes of cell-laden poly(D,L-lactic-co-glycolic acid) (PLGA) porous microspheres with thin encapsulation of agarose-collagen composite hydrogel (AC hydrogel). Highly porous microspheres enable cells to adhere and proliferate before printing. Meanwhile, AC hydrogel allows a...

Microfluidics for Biofabrication

Biography Shoji Takeuchi received his B.E., M.E., and Dr. Eng. degrees in mechanical engineering from the University of Tokyo, Tokyo, Japan in 1995, 1997, and 2000, respectively. He is currently a professor in and the Director of the Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS) at the University of Tokyo. He has authored more...